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Reference intervals for select hematologic and plasma biochemical analytes of wild Lake Sturgeon ( A cipenser fulvescens ) from the S t. L awrence R iver in N ew Y ork
Author(s) -
DiVincenti Louis,
Wyatt Jeff,
Priest Heather,
Dittman Dawn,
Klindt Rodger,
Gordon David,
Preston Andrew,
Smith Thomas,
Bowman Colby
Publication year - 2013
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/vcp.12017
Subject(s) - sturgeon , lake sturgeon , acipenser , biology , reference range , medicine , zoology , chemistry , endocrinology , fish <actinopterygii> , fishery
Background Lake Sturgeon ( A cipenser fulvescens ) is a long‐lived freshwater fish distributed throughout the G reat L akes region and is a threatened species in N ew Y ork S tate. The species' unique life cycle makes it useful as an indicator of ecosystem health, and efforts to repatriate Lake Sturgeon to their historic range are underway. However, comprehensive hematologic and biochemical reference intervals that would be valuable for assessing the health status of individual animals have not been reported. Objectives The objective of this study was to determine normal hematologic and plasma biochemical reference intervals for healthy wild Lake Sturgeon. Methods Blood samples were collected from 52 wild Lake Sturgeon caught in gill nets in the S t. L awrence R iver. Heparinized whole blood and plasma samples were analyzed using standard techniques. Reference intervals were calculated using the robust method following elimination of outliers and B ox– C ox transformation of data. Results Hematologic reference intervals were as follows: PCV 17–38%, estimated WBC count 2740–23,150/μL, neutrophils 193–6121/μL, eosinophils 0–558/μL, other granulocytes/heterophils 0–488/μL, lymphocytes 1447–14,044/μL, and monocytes 55–1684/μL. Plasma biochemical reference intervals were as follows: aspartate aminotransferase 333–1746 U/L, calcium 1.85–2.80 mmol/L, chloride 95–123 mmol/L, creatine kinase 776–35,536 U/L, glucose 2.94–14.76 mmol/L, glutamate dehydrogenase 6–30 U/L, phosphate 2.03–5.81 mmol/L, potassium 2.34–4.24 mmol/L, sodium 122.9–151.1 mmol/L, total protein 2.0–4.4 g/dL, triglycerides 1.07–5.12 mmol/L, and uric acid 1–251 μmol/L. Conclusion Reference intervals reported here will be useful for health assessment of wild and repatriated Lake Sturgeon.