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Murine red blood cells from genetically distinct donors cross‐regulate when stored together
Author(s) -
Hay Ariel,
Howie Heather L.,
Waterman Hayley R.,
de Wolski Karen,
Zimring James C.
Publication year - 2017
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.14313
Subject(s) - green fluorescent protein , flow cytometry , transgene , red blood cell , wild type , microbiology and biotechnology , biology , chemistry , genetically modified mouse , gene , immunology , biochemistry , mutant
BACKGROUND Donor variability of red blood cell (RBC) storage has been observed in both humans and animal models. We utilized a strain of mice with RBCs known to store well (B6) and a strain known to store poorly (FVB) to test the hypothesis that RBCs affected the storage of other RBCs. STUDY DESIGN AND METHODS Five strains of mice were used: 1) transgenic B6 mice expressing green fluorescent protein (GFP) in their RBCs (GFP.B6), 2) wild‐type B6 mice, 3) wild‐type FVB mice, 4) F1 crosses between GFP.B6 and FVB mice (GFP.F1), and 5) the analogous wild‐type (B6xFVB) F1 cross. GFP.B6 or GFP.F1 RBCs were mixed with wild‐type (non‐GFP) RBCs from B6 or FVB strains before storage. Twenty‐four–hour RBC recoveries were determined for stored RBCs by enumerating circulating GFP+ RBCs by flow cytometry. RESULTS Twenty‐four–hour recoveries of GFP.F1 RBCs was increased by co‐storage with B6 RBCs but decreased by co‐storage with FVB RBCs. This effect was dose dependent when tested with GFP.B6 RBCs; the more FVB blood added, the worse the 24‐hour recoveries became. RBC cross‐regulation did not occur when B6 and FVB RBCs were separated by a semipermeable membrane with a 0.4‐µm size cutoff. CONCLUSION These findings demonstrate that RBCs affect the storage of other RBCs, in both positive and negative directions, indicating not only that RBC storage is intrinsic to the RBC but that RBC–RBC communication occurs. Additional studies will be required to determine the nature of this effect and if these findings translate into human RBC storage.

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