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Evaluation of the quality of blood components obtained after automated separation of whole blood by a new multiunit processor
Author(s) -
Lagerberg Johan W.,
SaladoJimena Jose A.,
Löf Helena,
Bontekoe Ido J.,
Nielsen Connie,
Verheggen Caroline,
Waeg Geert,
Meer Pieter F.,
Korte Dirk,
Hansen Morten B.,
Knutson Folke
Publication year - 2013
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.12010
Subject(s) - blood product , hemolysis , blood component , whole blood , chemistry , in vivo , platelet , red blood cell , blood units , chromatography , andrology , biomedical engineering , medicine , blood transfusion , surgery , immunology , biology , biochemistry , microbiology and biotechnology , emergency medicine
Background The R eveos system ( T erumo BCT ) is a fully automated device able to process four whole blood ( WB ) units simultaneously into a plasma unit, a red blood cell ( RBC ) unit, and an interim platelet ( PLT ) unit ( IPU ). Multiple IPU s can be pooled to form a transfusable PLT product. The aim of our study was to evaluate the quality of components made with the Reveos system from either fresh (2‐8 hr) or overnight‐held WB . Study Design and Methods A prototype of the Reveos system was used to process WB . RBC s were resuspended in SAGM , leukoreduced, and assayed for in vitro quality variables during a 42‐day storage period at 2 to 6° C . Twenty‐four‐hour in vivo recovery was determined on Day 42. Plasma was assayed for cellular contamination and activation variables. IPU s were pooled with SSP + additive solution for in vitro quality assessments during a 7‐day storage period at room temperature. Results Reveos‐produced RBC s and plasma units met the predefined requirements. RBC recovery was superior to control units. On Day 42, hemolysis was below 0.8% and in vivo recovery was above 75% for all RBC s. Cellular contamination was lower for Reveos‐produced plasma. PLT yield was higher with overnight‐stored WB . PLT quality was well maintained during storage with no significant differences between the two groups. Conclusion Blood components prepared with the Reveos from fresh or overnight‐held WB meet quality criteria without any relevant difference between the two groups. The Reveos system has the potential to increase efficacy and standardization of blood component preparation.