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Recombination suppression in heterozygotes for a pericentric inversion induces the interchromosomal effect on crossovers in Arabidopsis
Author(s) -
Termolino Pasquale,
Falque Matthieu,
Aiese Cigliano Riccardo,
Cremona Gaetana,
Paparo Rosa,
Ederveen Antoine,
Martin Olivier C.,
Consiglio Federica M.,
Conicella Clara
Publication year - 2019
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.14505
Subject(s) - chiasma , meiosis , chromosomal inversion , chromosomal crossover , biology , genetics , synapsis , recombination , homologous chromosome , genetic recombination , homologous recombination , chromosome , heterozygote advantage , allele , mutant , gene , karyotype
Summary During meiosis, recombination ensures allelic exchanges through crossovers ( CO s) between the homologous chromosomes. Advances in our understanding of the rules of CO s have come from studies of mutations including structural chromosomal rearrangements that, when heterozygous, are known to impair CO s in various organisms. In this work, we investigate the effect of a large heterozygous pericentric inversion on male and female recombination in Arabidopsis. The inversion was discovered in the Atmcc1 mutant background and was characterized through genetic and next‐generation sequencing analysis. Reciprocal backcross populations, each consisting of over 400 individuals, obtained from the mutant and the wild type, both crossed with Landsberg erecta , were analyzed genome‐wide by 143 single‐nucleotide polymorphisms. The negative impact of inversion became evident in terms of CO loss in the rearranged chromosome in both male and female meiosis. No single‐ CO event was detected within the inversion, consistent with a post‐meiotic selection operating against unbalanced gametes. Cytological analysis of chiasmata in F 1 plants confirmed that CO s were reduced in male meiosis in the chromosome with inversion. Crossover suppression on the rearranged chromosome is associated with a significant increase of CO s in the other chromosomes, thereby maintaining unchanged the number of CO s per cell. The CO pattern observed in our study is consistent with the interchromosomal ( IC ) effect as first described in Drosophila . In contrast to male meiosis, in female meiosis no IC effect is visible. This may be related to the greater strength of interference that constrains the CO number in excess of the minimum value imposed by CO assurance in Arabidopsis female meiosis.