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Long‐term in vitro persistence of magnetic properties after magnetic bead‐based cell separation of T cells
Author(s) -
Laghmouchi Aicha,
Hoogstraten Conny,
Falkenburg J. H. Frederik,
Jedema Inge
Publication year - 2020
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/sji.12924
Subject(s) - cell division , in vitro , magnetic bead , cell , cell sorting , magnetic nanoparticles , magnetic separation , microbiology and biotechnology , cell culture , immunomagnetic separation , bead , lysis , biophysics , chemistry , biology , immunology , materials science , nanotechnology , nanoparticle , chromatography , genetics , biochemistry , metallurgy , composite material
Abstract Magnetic‐activated cell sorting (MACS) using magnetic nanoparticles coated with specific antibodies is commonly used in immunology research. For in vitro isolation purposes, it is important to know to what extent the magnetic properties remain present in the isolated cell populations and whether it has consequences for sequential isolations. We hypothesized that only upon cell division, cells will lose their magnetic properties via dilution of the particles in/on their daughter cells. We analysed residual magnetic properties of cells that divided vs cells that did not divide after magnetic bead‐based cell separation. As a model, we isolated T cells using beads targeting the non‐modulating surface molecule CD45RO. Cells were labelled with the cell division tracking dye PKH and cultured under different conditions to induce variable degrees of cell division. We demonstrate that T cells that underwent no, or only minimal, cell divisions after MACS retained magnetic properties for up to at least 2 weeks of in vitro culture. The presence of nanoparticles was detected on their cell surface and intracellularly using Labeling Check reagent. These results have important consequences for procedures requiring repetitive isolation rounds after in vitro culture.