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UVA irradiation of the eye modulates the contact hypersensitivity of the skin and intestines by affecting mast cells in mice
Author(s) -
Yamate Yurika,
Hiramoto Keiichi,
Kasahara Emiko,
Sato Eisuke F
Publication year - 2015
Publication title -
photodermatology, photoimmunology and photomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.736
H-Index - 60
eISSN - 1600-0781
pISSN - 0905-4383
DOI - 10.1111/phpp.12157
Subject(s) - oxazolone , hapten , fluorescein isothiocyanate , sensitization , immunology , chemistry , irradiation , epidermis (zoology) , mast cell , microbiology and biotechnology , medicine , antibody , biology , anatomy , physics , quantum mechanics , nuclear physics , fluorescence
Abstract Background Ultraviolet A ( UVA ) irradiation before allergic sensitization induces immunosuppression, but the precise mechanism remained unclear. In this study, we examined the influence of UVA irradiation of the eye on contact hypersensitivity ( CHS ) and the role of mast cells in CHS . Methods We used two types of haptens, fluorescein isothiocyanate ( FITC : a T h2 type hapten) and 4‐ethoxymethylene‐2‐phenyl‐2‐oxazolin‐5‐one (oxazolone: a T h1 type hapten). A 300 kJ/m 2 dose of UVA irradiation was delivered to the eyes. After UVA irradiation, we sensitized abdominal shaved skin and challenged the ear epidermis and colons of these mice with each hapten. Results After UVA irradiation, the CHS of the skin and colon were not inhibited in the FITC ‐sensitized mice. However, in the oxazolone‐sensitized mice, only the CHS of the skin was inhibited by UVA irradiation. The inflammation of the colon became more severe after UVA irradiation. In mast cell–deficient ( W / W v) mice sensitized to FITC , the CHS was weaker than that in WT mice. Moreover, the reduction of immunosuppression in ear swelling was seen for one of the two models they used. Conclusions These results suggest that the mast cells induced by UVA irradiation of the eye have different roles in the epidermis and colon and have different responses to different haptens.