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Vergleichende Bewertung des Premier R Enzymimmunassays, der Mikroimmundiffusion und der Komplementbindungsreaktion zum Nachweis von Antikörpern gegen Histoplasma capsulatum var. capsulatum
Author(s) -
Sekhon A. S.,
Kaufman L.,
Kobayashi G. S.,
Moledi.,
Jalbert M.,
Notenboom R. H.
Publication year - 1994
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.1994.37.9-10.313
Subject(s) - complement fixation test , immunoassay , immunodiffusion , histoplasma capsulatum , antibody , histoplasma , complement (music) , microbiology and biotechnology , histoplasmosis , chromatography , virology , chemistry , biology , immunology , serology , biochemistry , complementation , gene , phenotype
Summary A total of 178 sera, including 68 from proven cases of histoplasmosis (65 positive for the presence of Histoplasma capsulatum var. capsulatum antibodies and three positive for antigen), 93 from patients with suspected histoplasmosis but with no laboratory evidence of H. capsulatum var. capsulatum infection, 14 from humans with heterologous fungal and non‐fungal infections and three from normal individuals, were tested for IgG H. capsulatum antibodies and M or M and H precipitins by enzyme immunoassay (EIA) (Meridian Diagnostics, Cincinnati, OH, USA) and microimmunodiffusion (MID) respectively. Sixty‐three of the 68 histoplasmosis case sera demonstrated IgG antibody, and 65 of 68 demonstrated the presence of specific precipitins in the MID test. Nine positive case sera, when tested with the Laboratory Branch complement fixation (LBCF) test, reacted positively to whole yeast and histoplasmin antigens (titres 1 : 8 to 1 : 512). Three histoplasmosis case sera repeatedly tested negative for IgG, specific precipitins and complement‐fixing antibodies, whereas they were positive for Histoplasma antigen. Eighteen of 95 sera from patients without evidence of histoplasmosis demonstrated IgG antibody in the EIA only. Among these positive sera, three out of three cases of aspergillosis and three out of five cases of blastomycosis were confirmed. Sera from HIV‐infected and healthy individuals did not show IgG or M and/or H antibodies to H. capsulatum . Ninety‐three sera were negative by both EIA and MID. The EIA for IgG was less sensitive (97%) than MID (100%). The specificity of EIA and MID was 84% and 100% respectively.