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The atypical response regulator HP 1021 controls formation of the H elicobacter pylori replication initiation complex
Author(s) -
Donczew Rafał,
Makowski Łukasz,
Jaworski Paweł,
Bezulska Martyna,
Nowaczyk Małgorzata,
ZakrzewskaCzerwińska Jolanta,
ZawilakPawlik Anna
Publication year - 2015
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/mmi.12866
Subject(s) - biology , dna replication , response regulator , regulator , seqa protein domain , dna , chromosome , genetics , gene , dna binding protein , replication timing , microbiology and biotechnology , origin of replication , bacterial protein , transcription factor
Summary The replication of a bacterial chromosome is initiated by the D na A protein, which binds to the specific chromosomal region ori C and unwinds duplex DNA within the DNA ‐unwinding element ( DUE ). The initiation is tightly regulated by many factors, which control either D na A or ori C activity and ensure that the chromosome is duplicated only when the conditions favor the survival of daughter cells. The factors controlling ori C activity often belong to the protein families of two‐component systems. Here, we found that H elicobacter pylori ori C activity is controlled by HP 1021, a member of the atypical response regulator family. HP 1021 protein specifically interacts with H . pylori ori C at HP 1021 boxes (5′‐ TGTT [ TA ] C [ TA ]‐3′), which overlap with three modules important for ori C function: D na A boxes, the hypersensitivity (hs) region and the DUE . Consequently, HP 1021 binding to ori C precludes D na A ‐ ori C interactions and inhibits DNA unwinding at the DUE . Thus, HP 1021 constitutes a negative regulator of the H . pylori orisome assembly in vitro . Furthermore, HP 1021 boxes were found upstream of at least 70 genes, including those encoding C ag A and F ur proteins. We postulate that HP 1021 might coordinate chromosome replication, and thus bacterial growth, with other cellular processes and conditions in the human stomach.