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B acillus thuringiensis C ry6 A exhibits nematicidal activity to C aenorhabditis elegans bre mutants and synergistic activity with C ry5 B to C . elegans
Author(s) -
Yu Z.,
Luo H.,
Xiong J.,
Zhou Q.,
Xia L.,
Sun M.,
Li L.,
Yu Z.
Publication year - 2014
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12219
Subject(s) - bacillus thuringiensis , caenorhabditis elegans , mutant , biology , caenorhabditis , cross resistance , bacillales , microbiology and biotechnology , bacteria , genetics , bacillus subtilis , gene
Abstract The group of nematicidal crystal protein C ry6 A shares very low identity and exhibits different structure with C ry5 B , another well‐studied group of nematicidal crystal protein produced by B acillus thuringiensis . In this study, we assayed the susceptibility of bre mutants ( C aenorhabditis elegans with resistance to C ry5 B ) to C ry6 A a2 and examined the synergistic activity between C ry6 A a2 and C ry5 B a2. Our results show that all bre mutants are susceptible to C ry6 A a2 on the lethal activity, growth inhibition, fertility and exhibit no cross‐resistance to C ry6 A a2. Moreover, all combinations of C ry6 A a2 and C ry5 B a2 with serial ratios exhibit significant synergism to C . elegans , and the highest synergistic effect was observed when C ry6 A a2 and C ry5 B a2 were mixed with a ration of 4 : 1. The susceptibility of bre mutants to C ry6 A and synergistic activity between C ry6 A and C ry5 B may be attributed to the diverse action mode, because of different structure of the two nematicidal crystal protein toxins. Significance and Impact of the Study B acillus thuringiensis nematicidal crystal proteins C ry6 A and C ry5 B share notable structural differences. Our study confirmed that there is no cross‐resistance of C aenorhabditis elegans bre mutants (with resistance to C ry5 B ) to C ry6 A , and combination of C ry6 A and C ry5 B shows synergistic activity against C . elegans . This result provides a more effective strategy for biocontrol of plant‐parasitic nematodes using a combination of C ry6 A and C ry5 B . Combination of C ry6 A and C ry5 B could enhance nematicidal toxicity of B t and supply an alternative to overcome the potential resistance of plant‐parasitic nematodes when large‐scale and long‐term use of B t toxins.