Open AccessSerum Thymidine Kinase 1, Canine‐C‐Reactive Protein, Haptoglobin, and Vitamin D Concentrations in Dogs with Immune‐Mediated Hemolytic Anemia, Thrombocytopenia, and Polyarthropathy
Author(s) -
Grobman M.,
Outi H.,
Rindt H.,
Reinero C.
Publication year - 2017
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/jvim.14787
Subject(s) - medicine , haptoglobin , gastroenterology , c reactive protein , hemolytic anemia , hematocrit , biomarker , immunology , inflammation , biochemistry , chemistry
Background Relapses of immune‐mediated hemolytic anemia ( IMHA ), thrombocytopenia ( ITP ), or polyarthropathy ( IMPA ) occur despite normal hematologic and cytologic parameters. Thymidine kinase 1 ( TK 1), canine C‐reactive protein (c‐ CRP ), haptoglobin ( HPT ), and 25‐Hydroxyvitamin‐ D (25( OH )D) might be adjunct to current monitoring strategies. Hypothesis/Objectives Compare serum concentrations of TK 1, c‐ CRP , HPT , and 25( OH )D in dogs with well‐ and poorly controlled primary IMHA , ITP , or IMPA . Animals Thirty‐eight client‐owned dogs. Methods Prospective descriptive study. Dogs diagnosed with IMHA , ITP , or IMPA had serum biomarker concentrations measured commercially. Disease control was assessed by hematocrit/ PCV and reticulocyte count, platelet count, and synovial fluid cytology for IMHA , ITP , and IMPA , respectively. Statistical analysis performed by Mann‐Whitney rank‐sum tests and receiver operating characteristic curves. Results TK 1 and c‐ CRP , but not HPT significantly decreased with well‐ versus poorly controlled IMHA ( P = 0.047, P = 0.028, P = 0.37). C‐ CRP , but not TK or HPT was significantly lower with well‐ versus poorly controlled IMPA ( P = 0.05, P = 0.28, P = 0.84). Sensitivity and specificity of TK and c‐ CRP (simultaneously) for detecting dogs with poorly controlled IMHA were 88 and 100%, respectively. Sensitivity and specificity of c‐ CRP for detecting poorly controlled dogs with IMPA were 13 and 100%, respectively. 92% of dogs were vitamin D insufficient (<100 ng/mL) regardless of disease control. Conclusions and Clinical Importance Combining TK 1 and c‐ CRP might act markers of disease control in dogs with IMHA . Canine‐ CRP cannot be recommended as an independent marker of disease control in IMPA . 25( OH )D insufficiency in immune‐mediated disorders might benefit from further study to determine if supplementation could improve therapeutic response or reduce disease risk.