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Inducible expression of A Disintegrin and Metalloproteinase 8 in chronic periodontitis and gingival epithelial cells
Author(s) -
Aung W. P. P.,
Chotjumlong P.,
Pata S.,
Montreekachon P.,
Supanchart C.,
Khongkhunthian S.,
Sastraruji T.,
Krisanaprakornkit S.
Publication year - 2017
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12426
Subject(s) - fusobacterium nucleatum , chronic periodontitis , periodontitis , metalloproteinase , downregulation and upregulation , immunohistochemistry , medicine , porphyromonas gingivalis , chemistry , matrix metalloproteinase , gene , biochemistry
Background and Objectives The expression of A Disintegrin and Metalloproteinase 8 ( ADAM 8) is associated with several inflammatory diseases. Elevated ADAM 8 levels have been shown in gingival crevicular fluid of patients with chronic periodontitis. The objective of this study was to investigate ADAM 8 expression in chronic periodontitis tissues compared with that in normal tissues. ADAM 8 expression and its inductive mechanism were examined in human gingival epithelial cells ( HGEC s) and human gingival fibroblasts. Material and Methods Total RNA and protein were extracted from gingival biopsies of 33 patients with chronic periodontitis and those of 23 healthy volunteers. ADAM 8 mRNA and protein expression was analyzed by real‐time polymerase chain reaction, immunoblotting and immunohistochemistry. ADAM 8 expression in control and stimulated cells in the presence or absence of specific inhibitors for mitogen‐activated protein kinase pathways was assayed by real‐time polymerase chain reaction, immunoblotting, flow cytometry and immunofluorescence. Results ADAM 8 mRNA and protein expression in chronic periodontitis tissues was significantly greater than that in normal tissues ( p < 0.01). Significantly increased ADAM 8 expression was detected in the gingival epithelium of chronic periodontitis tissues ( p < 0.001). ADAM 8 mRNA expression in HGEC s, but not in human gingival fibroblasts, was significantly induced by stimulation with Fusobacterium nucleatum ( p < 0.05), partially via the p44/42 mitogen‐activated protein kinase pathway. ADAM 8 expression in the cell lysates and on the surface of HGEC s was induced by stimulation with F. nucleatum . Conclusion ADAM 8 expression is increased in inflamed chronic periodontitis tissues and localized within gingival epithelium, consistent with an upregulation of ADAM 8 expression in F. nucleatum ‐stimulated HGEC s, suggesting a possible role of ADAM 8 in innate immunity of periodontal tissue.