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Ectopic Expression of Rice O s BIANK 1 , Encoding an Ankyrin Repeat‐Containing Protein, in Arabidopsis Confers Enhanced Disease Resistance to B otrytis cinerea and P seudomonas syringae
Author(s) -
Li Dayong,
Wang Fengrang,
Liu Bo,
Zhang Yafen,
Huang Lei,
Zhang Huijuan,
Song Fengming
Publication year - 2013
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12023
Subject(s) - biology , botrytis cinerea , pseudomonas syringae , ankyrin repeat , arabidopsis , plant disease resistance , ectopic expression , r gene , gene , phytotoxin , transgene , botany , microbiology and biotechnology , genetics , mutant , toxin
Abstract Ankyrin repeat‐containing proteins comprise a large family whose members have been shown to play important roles in various aspects of biological processes in plant growth and development as well as in responses to biotic and abiotic stresses. We previously identified a rice gene, O s BIANK 1 , encoding an ankyrin repeat‐containing protein and found that expression of O s BIANK 1 can be induced by defence signalling molecules and by infection of M agnaporthe oryzae, the causal agent of blast disease. To better understand the possible function of O s BIANK 1 in disease resistance, we generated transgenic Arabidopsis plants that constitutively overexpress the O s BIANK 1 gene. Results from disease assays revealed that the O s BIANK 1 ‐overexpressing plants display increased resistance against Botrytis cinerea and P seudomonas syringae pv. tomato DC 3000 as compared with the wild‐type plants. In O s BIANK 1 ‐overexpressing plants, expression of some of well‐known defence genes (e.g. PR ‐ 1 , PR ‐ 2 and PDF 1.2 ) was up‐regulated after infection with B . cinerea or P . syringae pv. tomato DC 3000. Furthermore, the O s BIANK 1 ‐overexpressing plants showed decreased levels of reactive oxygen species (i.e. superoxide anion and H 2 O 2 ) after B otrytis infection. Thus, our present results further support the role of O s BIANK 1 in regulation of defence responses against different types of pathogens.