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Vinpocetine and α‐tocopherol prevent the increase in DA and oxidative stress induced by 3‐ NPA in striatum isolated nerve endings
Author(s) -
HerreraMundo Nieves,
Sitges María
Publication year - 2013
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/jnc.12082
Subject(s) - vinpocetine , chemistry , lipid peroxidation , oxidative stress , pharmacology , biochemistry , reactive oxygen species , neuroprotection , antioxidant , ascorbic acid , superoxide dismutase , respiratory chain , mitochondrion , biology , food science
Abstract Vinpocetine is a neuroprotective drug that exerts beneficial effects on neurological symptoms and cerebrovascular disease. 3‐nitropropionic acid (3‐ NPA ) is a toxin that irreversibly inhibits succinate dehydrogenase, the mitochondrial enzyme that acts in the electron transport chain at complex II. In previous studies in striatum‐isolated nerve endings (synaptosomes), we found that vinpocetine decreased dopamine ( DA ) at expense of its main metabolite 3,4‐dihydroxyphenylacetic acid ( DOPAC ), and that 3‐ NPA increased DA , reactive oxygen species ( ROS ), DA ‐quinone products formation, and decreased DOPAC . Therefore, in this study, the possible effect of vinpocetine on 3‐ NPA ‐induced increase in DA , ROS , lipid peroxidation, and DA ‐quinone products formation in striatum synaptosomes were investigated, and compared with the effects of the antioxidant α‐tocopherol. Results show that the increase in DA induced by 3‐ NPA was inhibited by both 25 μM vinpocetine and 50 μM α‐tocopherol. Vinpocetine, as α‐tocopherol, also inhibited 3‐ NPA ‐induced increase in ROS (as judged by DCF fluorescence), lipid peroxidation (as judged by TBA ‐ RS formation), and DA ‐quinone products formation (as judged by the nitroblue tetrazolium reduction method). As in addition to the inhibition of complex II exerted by 3‐ NPA , 3‐ NPA increases DA ‐oxidation products that in turn can inhibit other sites of the respiratory chain, the drop in DA produced by vinpocetine and α‐tocopherol may importantly contribute to their protective action from oxidative damage, particularly in DA ‐rich structures.