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Frequencies of lymphoid T‐follicular helper cells obtained longitudinally by lymph node fine‐needle aspiration correlate significantly with viral load in SIV ‐infected rhesus monkeys
Author(s) -
Klippert Antonina,
StolteLeeb Nicole,
Neumann Berit,
Sauermann Ulrike,
Daskalaki Maria,
Gawanbacht Ali,
Kirchhoff Frank,
StahlHennig Christiane
Publication year - 2015
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/jmp.12186
Subject(s) - flow cytometry , viral load , lymph node , lymph , population , simian immunodeficiency virus , t lymphocyte , biology , immunology , microbiology and biotechnology , pathology , virology , medicine , virus , immune system , environmental health
Abstract Background T‐follicular helper ( T FH ) cells are an important population in lymph nodes ( LN s) contributing to the generation of highly specific B cells. For SIV studies in rhesus macaques ( RM ), analysis of LN is necessary, but restricted due to invasive sampling. We applied the minimally invasive LN fine‐needle aspiration ( LN ‐ FNA ) and examined dynamics of T FH cells during SIV infection. Materials and methods LN ‐ FNA and LN resection were carried out on uninfected RM . Lymphocytes were analyzed by flow cytometry. Additionally, cells obtained by LN ‐ FNA over time from SIV ‐infected RM were analyzed. Results Percentages of lymphocyte subsets were similar in LN aspirates and whole LN s. Analysis of LN aspirates from SIV ‐infected RM demonstrated a decrease of CD 4 + T cells, while T FH cell frequencies increased over time and correlated significantly with plasma viral load. Conclusions By applying LN ‐ FNA , we showed that T FH cell expansion in chronic SIV infection is associated with viral load.