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Microbiological evidences for gastric cardiac microflora dysbiosis inducing the progression of inflammation
Author(s) -
Yan Ruijing,
Guo Yi,
Gong Qingyun,
Chen Man,
Guo Yuanning,
Yang Peng,
Huang Hao,
Huang Haihua,
Huang Wen,
Ma Zikai,
Zheng Zhijian,
Tian Dongping,
Su Min
Publication year - 2020
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/jgh.14946
Subject(s) - dysbiosis , inflammation , medicine , firmicutes , microbiology and biotechnology , microbiome , ruminococcus , bacteroides , immunology , biology , gut flora , bacteria , bioinformatics , 16s ribosomal rna , genetics
Abstract Background and Aim Nowadays, anti‐inflammation treatment is a promising approach for preventing tumorigenesis, and human microflora is closely related to inflammation. This study aimed to investigate the gastric cardiac microbiome and identify inflammation‐related microorganisms for gastric cardiac inflammation. Methods We performed 16S rRNA sequencing on a total of 11 healthy individuals and 89 individuals with different degree of gastric cardiac inflammation. Immunohistochemistry was used for verifying candidate bacteria. Phylogenetic reconstruction of unobserved states ( picrust ) was used for predicting the pathways involved by cardiac microflora. Results The resident phyla in normal were Proteobacteria , Firmicutes , Bacteroides , and Actinobacteria , and the dominant genus in normal were Halomonas , shewanella , and Comamonas . In the progression of gastric cardiac inflammation, the diversity of cardiac microflora did not change ( P > 0.05). However, the composition structure of cardiac microflora varied between healthy and inflamed tissues ( P < 0.05). Meanwhile, there were 64 species parallel increased with inflammation degree, especially Helicobacter pylori, Lactobacillus spp. Additionally, inflammation‐related species were detected ( P < 0.05), including H. pylori , Acinetobacter ursingii , and Streptococcus agalactiae . Higher H. pylori colonization was positively related to the progression of cardiac inflammation ( γ coefficient = 0.678, P < 0.001), and it also influenced the cardiac microbial community structure. Cardiac microflora also participated in DNA repair pathways and is affected by the relative abundance of H. pylori ( P < 0.0001). Conclusions Cardiac microflora dysbiosis, especially the increasing of the relevant abundance of H. pylori , promotes the progression of cardiac inflammation.