Premium
Isolation of Major Phenolic Compounds from the Extracts of P runella L . Species Grown in T urkey and Their Antioxidant and Cytotoxic Activities
Author(s) -
Şahin Saliha,
Ari Ferda,
Demir Cevdet,
Ulukaya Engin
Publication year - 2014
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.12043
Subject(s) - prunella vulgaris , rosmarinic acid , caffeic acid , biology , traditional medicine , botany , antioxidant , biochemistry , medicine , traditional chinese medicine , alternative medicine , pathology
Abstract The major phenolics of P runella L . species were isolated, and antioxidant activites were determined by 2,2‐azino‐di‐(3‐ethylbenzothialozine‐sulphonic acid). The isolated compounds were identified as rutin, rosmarinic acid and caffeic acid by high performance liquid chromatography‐diode array detection. The results show that the phenolic compounds were isolated from P runella L . species with high content ranged from 74% ( P runella grandiflora L .) to 99% ( P runella orientalis B ornm.), and the major compound of rosmarinic acid was isolated above 90% in all species. Antigrowth effects of P runella L . species were tested against human breast cancer cell lines, MCF‐7 (estrogen receptor‐positive) and MDAMB‐231 (estrogen receptor‐negative) by the MTT (3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazoliumbromide) and ATP (adenosine triphosphate) viability assays. To determine the mode of cell death, M 30‐antigen assay was performed in order to measure apoptosis. It was found that P runella laciniata ( L .) L ., P . orientalis B ornm. and P . grandiflora L . species showed antigrowth effect on MCF ‐7 and MDA‐MB ‐231 breast cancer cells, but apoptosis was not observed. Practical Applications Four P runella L . species ( P runella vulgaris L ., P runella laciniata L ., P runella grandiflora L . and P runella orientalis B ornm.) have been used in W estern and C hinese herbal medicine that possesses antioxidant properties. P runella L . species have a broad chemical composition, consisting of bioactive molecules as phenolic acids, flavonoids and anthocyanidins. It was found that P runella L . species showed a significant growth‐inhibiting effect on human breast cancer cells, but apoptosis was not involved in cell death. The results suggest that the isolated rutin and rosmarinic acid, as the principal components of P runella L . extracts, could be utilized as both medicine and herbal tea.