Premium
Assessment of the rate of solid‐phase gastric emptying in ponies by means of the 13 C‐octanoic acid breath test: a preliminary study
Author(s) -
WYSE C. A.,
MURPHY D. M.,
PRESTON T.,
MORRISON D. J.,
LOVE S.
Publication year - 2001
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/j.2042-3306.2001.tb00601.x
Subject(s) - breath test , gastric emptying , coefficient of variation , ingestion , meal , chemistry , sampling time , chromatography , medicine , nuclear medicine , gastroenterology , stomach , mathematics , statistics , helicobacter pylori
Summary The aim of this study was to assess the feasibility of applying the 13 C‐octanoic acid breath test for assessment of gastric emptying in ponies by investigating the pattern of 13 C enrichment in breath following the administration of a test meal ± 13 C‐octanoic acid. After a 14 h fast, the ponies received either no meal (Test I) or a standardised test meal labelled with 0 mg (Test II) , 125 mg (Test III) , 250 mg (Test IV) or 500 mg (Test V) 13 C‐octanoic acid. For each test (I‐V), exhaled breath samples were collected in duplicate at 1 h and immediately before ingestion of the test meal and at frequent intervals thereafter for 12 h. Breath samples were analysed by continuous flow isotope ratio mass spectrometry. Three indices of breath 13 C‐enrichment were computed; half dose recovery time (t 1/2), gastric emptying coefficient (GEC) and time to peak breath 13 C‐enrichment t(max). For Tests I and II , the ratio of 13 CO 2 : 12 CO 2 remained stable for the duration of the sampling period. For Tests III, IV and V , an increase in the ratio of 13 CO 2 : 12 CO 2 was detected. The test was reproducible within individuals, and intersubject variation was low. Further validation studies of this noninvasive technique are justified.