Open AccessEffect of Heart Failure on Dipeptidyl Peptidase IV Activity in Plasma of Dogs
Author(s) -
Gomez N.,
Matheeussen V.,
Damoiseaux C.,
Tamborini A.,
Merveille A.C.,
Jespers P.,
Michaux C.,
Clercx C.,
Meester I.,
Mc Entee K.
Publication year - 2012
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/j.1939-1676.2012.00942.x
Subject(s) - medicine , heart failure , beagle , dipeptidyl peptidase , natriuretic peptide , pathophysiology , cardiology , endocrinology , dilated cardiomyopathy , cardiomyopathy , circadian rhythm , enzyme , biochemistry , chemistry
Background In congestive heart failure ( HF ), plasma B‐type natriuretic peptide ( BNP ) seems devoid of biological effectiveness. BNP 1–32 could be truncated into BNP 3–32 by dipeptidyl peptidase IV ( DPP 4), and BNP 3–32 has reduced biological activities. Hypothesis Increased DPP 4 activity is associated with pathophysiology of HF . Animals One hundred twenty‐eight client‐owned dogs and 9 experimental Beagles from the C linical V eterinary U nit of the U niversity of L iège. Methods We prospectively measured plasma DPP 4 activity in 5 groups of dogs: normal growing dogs (n = 21), normal adult dogs (n = 60), healthy Beagle (n = 9), dogs with myxomatous mitral valve disease (n = 35), and dogs with dilated cardiomyopathy (n = 12). The final diagnosis and the severity of HF were determined by D oppler echocardiography. Plasma DPP 4 activity was measured kinetically by a fluorimetric method. Results In growing dogs, DPP 4 activity was higher than in adults ( P < .001) and inversely correlated with age ( r = −0.57, P < .01). In adults, DPP 4 activity increased linearly with body weight ( r = 0.39, P < .01), but there was no influence of age or sex. No effect of the circadian rhythm was noted. DPP 4 activity was significantly higher in HF ISACHC I (16.3 ± 1.14 U/L) compared with healthy adults (12.4 ± 0.65 U/L, P < .05) and HF ISACHC III (11.0 ± 1.50 U/L, P < .05). Mean DPP 4 activity in ISACHC II was 15.1 ± 1.4 U/L. Conclusion and Clinical Importance We did not find evidence that plasma DPP 4 activity is responsible for the “ BNP resistance” in overt congestive HF , but it may be implicated in early stages.