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Establishment of canine hematology reference intervals for the S ysmex XT ‐2000iV hematology analyzer using a blood donor database
Author(s) -
Serra Mariana,
Freeman Kathleen P.,
Campora Carola,
Sacchini Federico
Publication year - 2012
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.2012.00417.x
Subject(s) - hematology analyzer , medicine , hematology , complete blood count
Background The S ysmex XT ‐2000iV is a hematology analyzer that combines impedance and optical techniques and has been previously validated for dogs. Specific reference intervals ( RI s) are useful when interpreting results. Objectives The aim of this study was to determine hematologic RI s for the S ysmex XT ‐2000iV using a large reference population of client‐owned clinically healthy blood donor dogs, adopting an indirect sampling method. Methods Dogs were screened for breed, size, health, travel history, and previous blood transfusions, and the quality of blood specimens was also reviewed. Results from specimens that met inclusion criteria were used to determine RI s using a nonparametric method. Specimens from A kitas and s ighthounds were excluded from the study. Results Of 992 specimens that had been collected from blood donors and analyzed, 297 were initially included in the RI study. An additional 38 specimens were excluded as outliers, and hematologic RI s for the S ysmex XT ‐2000iV were based on analysis of specimens from 259 clinically healthy dogs. Measurands evaluated had variable distributions, and intervals obtained were generally comparable to previously reported RI s. Differences observed included higher lower and upper reference limits ( LRL and URL , respectively) for MCV and lower URL for WBC count. Reticulocyte count and the LRL of the absolute lymphocyte count were also higher than previously reported, and the RI for platelet count was narrower and lower. Conclusions Canine RI s for the S ysmex hematology analyzer were established using an indirect sampling method with reference individuals selected from a large database of client‐owned clinically healthy blood donor dogs. For specimens included in this study, time from collection to analysis was similar to what veterinary commercial laboratories experience.