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Development and Clinical Evaluation of A PCR Assay Targeting the Metalloprotease Gene ( mprA ) of B. pseudomallei
Author(s) -
Neubauer H.,
Sprague L. D.,
Joseph M.,
Tomaso H.,
Al Dahouk S.,
Witte A.,
Kinne J.,
Hensel A.,
Wernery R.,
Wernery U.,
Scholz H. C.
Publication year - 2007
Publication title -
zoonoses and public health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.87
H-Index - 65
eISSN - 1863-2378
pISSN - 1863-1959
DOI - 10.1111/j.1863-2378.2007.01008.x
Subject(s) - burkholderia pseudomallei , biology , microbiology and biotechnology , melioidosis , burkholderia , polymerase chain reaction , gene , virology , bacteria , genetics
Summary A PCR assay targeting the metalloprotease gene ( mprA ) of Burkholderia pseudomallei was developed for the specific detection of this organism in pure cultures and clinical samples. All other closely related organisms including B. mallei the causative agent of glanders, and B. thailandensis tested negative. Burkholderia pseudomallei DNA was successfully amplified from paraffin‐embedded lung tissue of a camel with a generalized B. pseudomallei infection. The developed PCR assay can be used as a simple tool for the specific and sensitive detection of B. pseudomallei .