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Anti‐fibronectin serum inhibits the disorganization of the dermal‐epidermal junction in cultured wounded skin
Author(s) -
Denefle JeanPierre,
Zhu QuianLong,
Lechaire JeanPierre
Publication year - 1989
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1989.tb03013.x
Subject(s) - fibronectin , lamina densa , biology , immunoperoxidase , dermis , dermoepidermal junction , epidermis (zoology) , basal lamina , cytoplasm , stratum spinosum , microbiology and biotechnology , staining , pathology , anatomy , stratum corneum , ultrastructure , extracellular matrix , immunology , monoclonal antibody , antibody , genetics , medicine
Summary— During wound‐healing in cultured frog skin fragments, fibronectin (FN) was detected in the dermal‐epidermal junction. Intracellular fibronectin was stained using permeabilization and DAB immunoperoxidase. With electron microscopy intracytoplasmic FN granules were localized in the epidermal processes of the stratum germinativum cells protruding towards the dermis and in their marginal regions (membrane‐associated plaques). Faint staining was visible at the level of the lamina densa and inside some parts of the lamina lucida. In comparison, contrasted ultrathin sections revealed classical disorganization of the dermal‐epidermal junction. In the presence of anti‐fibronectin serum during the whole time of culture, fibronectin‐antifibronectin binding was visualized in the form of sparse cytoplasmic granules in the epidermal processes of the stratum germinativum cells. Contrasted ultrathin sections emphasized the continuity between the tonofilaments, the anchoring filaments and the anchoring fibrils. Briefly, anti‐fibronectin serum inhibits the disorganization of the dermal‐epidermal junction in cultured wounded skin.