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Ocular hypertension in adult rodents does not affect non‐ RGC neurons in the ganglion cell layer but results in severe loss of cone‐photorreceptors
Author(s) -
VidalSanz M.,
RodríguezLlarena S.,
OrtínMartínez A.,
SalinasNavarro M.,
NadalNicolás F.M.,
ValienteSoriano F.J.,
VillegasPérez M.P.
Publication year - 2015
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2015.0119
Subject(s) - dapi , ocular hypertension , retina , ganglion cell layer , retinal , ophthalmology , ganglion , retinal ganglion cell , population , biology , anatomy , pathology , staining , medicine , neuroscience , intraocular pressure , environmental health
Summary We investigate short and long‐term effects of laser photocoagulation ( LP ) of the limbal tissues‐induced ocular hypertension ( OHT ) in the innermost and outer layers of the retina ( OLR ). Adult albino rats or mice were examined 2 weeks to 6 months after LP ‐induced OHT . Brn3a‐immunodetection was used to identify retinal ganglion cells ( RGC s) and DAPI ‐staining to identify all cell nuclei in the ganglion cell layer. Retinas were cut in cross sections for morphometric analysis or prepared as wholemounts to study the entire population of RGC s or L‐ and S‐cones (immunolabeled). OHT resulted in pie‐shaped retinal areas lacking Brn3a + RGC s but with large numbers of DAPI + nuclei. Cross‐sections showed focal regions of degeneration affecting the ORL that became evident by 2 m and progressed up to 6 months after LP . Rat retinal wholemounts showed RGC diminished to 20–25% by 1 m with no further loss, whereas the L‐ or S‐cones showed progressive loss up to 6 months that amounted to 19% or 33% by 1 m, to 62% or 51% by 3 m, and to 66% or 59% by 6 m, respectively. LP ‐induced OHT results in selective loss of RGC s within the ganglion cell layer, but there is progressive severe damage of the ORL up to 6 m.