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Expression of 12 cytokines in aqueous humour of uveal melanoma before and after combined Ruthenium‐106 brachytherapy and transpupillary thermotherapy
Author(s) -
Lee Christopher Seungkyu,
Jun Ik Hyun,
Kim Taeim,
Byeon Suk Ho,
Koh Hyoung Jun,
Lee Sung Chul
Publication year - 2012
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2012.02392.x
Subject(s) - medicine , aqueous humour , melanoma , brachytherapy , vascular endothelial growth factor , tumor necrosis factor alpha , cytokine , ophthalmology , pathology , aqueous humor , cancer research , vegf receptors , radiation therapy
. Purpose: To determine the aqueous humour levels of 12 cytokines in eyes with uveal melanoma and whether their expression changes after combined Ru‐106 brachytherapy and transpupillary thermotherapy (TTT). Methods: Aqueous humour samples were collected from 20 patients with previously untreated uveal melanoma undergoing combined Ru‐106 brachytherapy and TTT, both at the time of plaque placement and removal. Using multiplex biochip array technology, 12 different cytokines were measured, including interleukin (IL)‐1α, IL‐1β, IL‐2, IL‐4, IL‐6, IL‐8, IL‐10, vascular endothelial growth factor (VEGF), tumour necrosis factor (TNF)‐α, interferon (IFN)‐γ, epidermal growth factor (EGF) and monocyte chemoattractant protein (MCP)‐1. Aqueous humour from 20 patients undergoing cataract surgery was used as control. Results: IL‐6, IL‐8, IFN‐γ and MCP‐1 were highly expressed in uveal melanoma, whereas IL‐2, IL‐10 and TNF‐ α were low in expression. There was a positive correlation between tumour height and IL‐8 level (p = 0.020). Vascular endothelial growth factor tends to be highly expressed in melanoma‐containing eyes (p = 0.056). Levels of IL‐6, IL‐8 and IL‐1β increased after the mean 117 ± 38 hrs of brachytherapy and adjunctive TTT with a tumour apex dose of 61 ± 28 Gy and a scleral contact dose of 786 ± 226 Gy. Increase in levels of IL‐6 (p = 0.003) and IL‐8 (p = 0.046) positively correlated with scleral contact dose. Conclusions: Cytokines such as IL‐6, IL‐8, IFN‐γ and MCP‐1 may be implicated in the progression of uveal melanoma. Ocular irradiation from a Ru‐106 plaque promoted an increase in the levels of IL‐6, IL‐8 and IL‐1β, modulation of which could be useful in managing radiation‐related side effects.