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Influence of glucose, fructose and aldose reductase inhibition on retinal sorbitol metabolism
Author(s) -
Naeser Peter,
Brolin Sven E.
Publication year - 1991
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1991.tb04845.x
Subject(s) - sorbitol , fructose , incubation , aldose reductase , aldose reductase inhibitor , sorbitol dehydrogenase , chemistry , polyol pathway , medicine , endocrinology , biochemistry , biology , enzyme
Abstract The sorbitol shunt has been studied in bovine retinal tissue at incubation times from 1 to 24 h. It was shown that an elevated glucose concentration (22 mM) of the medium was accompanied by a slight increase in sorbitol content already after 3 h. At longer incubation times, but lower glucose concentration (11.1 mM) there was a similar increase. Addition of an aldose reductase (AR) inhibitor prevented the sorbitol increase. Addition of fructose to the medium significantly increased the sorbitol accumulation above the effet seen with glucose alone and this effect was not influenced by the AR inhibitor. Thus the sorbitol concentration in the retina may be increased after a short incubation time and further enhanced by the presence of fructose.