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Development of microsatellites for Scirpus mariqueter Wang et Tang (Cyperaceae) and cross‐species amplification in Scirpus planiculmis F. Schmidt
Author(s) -
ZHOU YEBIN,
WANG HAO,
YANG MEI,
CHEN JIAKUAN,
ZHANG WENJU
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02453.x
Subject(s) - biology , microsatellite , cyperaceae , scirpus , loss of heterozygosity , population , botany , genetics , ecology , gene , allele , wetland , poaceae , demography , sociology
We report eight microsatellite markers developed for Scirpus mariqueter Wang et Tang using 5′‐anchored PCR. They were tested using 30 S. mariqueter individuals. The observed and expected heterozygosity levels ranged from 0.0690 to 0.8426 and from 0.0678 to 0.5249, respectively. Cross‐amplification was carried out in a congener, S. planiculmis F. Schmidt. All these markers will be very helpful in population genetics study of S. mariqueter and S. planiculmis.