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Novel primers for the amplification of nuclear DNA introns in the entomopathogenic nematode Heterorhabditis bacteriophora and their cross‐amplification in seven other Heterorhabditis species
Author(s) -
REGEAI S. O.,
DOLAN K. M.,
FITZPATRICK D. A.,
BROWNE J. A.,
JONES J. T.,
BURNELL A. M.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02431.x
Subject(s) - biology , heterorhabditis bacteriophora , genetics , intron , entomopathogenic nematode , nematode , population , gene , ecology , demography , sociology
We describe 24 novel primers that amplify intron regions in housekeeping and structural genes of Heterorhabditis bacteriophora . The cross‐amplification potential of these primers in seven other Heterorhabditis species was determined. The results obtained showed interspecific nucleotide, length and splice site variability in the sequenced introns and for one gene, an intron gain was observed. These primers will be useful tools for studying population genetics, genetic diversity and intron DNA evolution within the genus Heterorhabditis and other genera of rhabditid nematodes.