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Isolation and characterization of microsatellite markers from the limpet Cellana grata
Author(s) -
NG W. C.,
CHAN M. N.,
SLINGSBY G.,
WILLIAMS GRAY A.,
LEUNG F. C. C.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02418.x
Subject(s) - biology , limpet , microsatellite , loss of heterozygosity , genetic variation , population , allele , zoology , genetic structure , genetics , ecology , intertidal zone , gene , demography , sociology
Abstract This note describes the development of nine polymorphic microsatellite loci in the limpet Cellana grata to investigate population structure and cohort variation in this species. The number of alleles ranged from seven to 22 and observed heterozygosity ranged from 0.62 to 0.95. Deviation from the Hardy–Weinberg equilibrium was detected in two loci, both as a result of heterozygote deficiency. Null alleles were detected in one of these loci. These genetic markers will be used to investigate the genetic structure of C. grata populations, as well as variation among cohorts of this common intertidal species.