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Isolation, characterization and cross‐species testing of microsatellites obtained from a sand smelt ( Atherina boyeri ) genomic library
Author(s) -
MILANA VALENTINA,
SOLA LUCIANA,
ROSSI ANNA R.,
BARBISAN FEDERICA,
CONGIU LEONARDO
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02366.x
Subject(s) - biology , microsatellite , zoology , isolation (microbiology) , smelt , amplified fragment length polymorphism , genetics , evolutionary biology , fishery , allele , gene , fish <actinopterygii> , bioinformatics , genetic diversity , population , demography , sociology
Abstract This study reports the isolation and characterization of 11 polymorphic microsatellites from a sand smelt ( Atherina boyeri ) genomic library. Enrichment was performed with di‐, tri‐ and tetranucleotide motifs following the FIASCO procedure (fast isolation by AFLP of sequences containing repeats). All loci were found to be in linkage and in Hardy–Weinberg equilibrium. This represents the first microsatellite isolation for the family Atherinidae and the isolated loci were accordingly tested on four additional species of the family: two recognized ( A. presbyter and A. hepsetus ) and two proposed (‘punctata’ and ‘non‐punctata’ forms). Moreover their cross‐species suitability on Menidia menidia , belonging to the same order but to the family Atherinopsidae, was also tested.