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Microsatellite markers for the fungal banana pathogens Mycosphaerella fijiensis , Mycosphaerella musicola and Mycosphaerella eumusae
Author(s) -
ZAPATER MF.,
DUCHEMIN M.,
DUSSART JF.,
COSTE D.,
BROTTIER P.,
CARLIER J.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02180.x
Subject(s) - mycosphaerella , biology , leaf spot , botany , microsatellite , multiplex polymerase chain reaction , septoria , polymerase chain reaction , genetics , gene , allele
Abstract We developed a total of 50 microsatellite markers for the three fungal pathogens causing the most important leaf spot diseases of banana: 32 loci for Mycosphaerella fijiensis are presented, and nine loci each for Mycosphaerella musicola and Mycosphaerella eumusae . All these loci were polymorphic within each species on a sample of isolates collected from various locations around the world. Within M. fijiensis and M. musicola , most of the loci tested (> 80%) in a sample of isolates from a single location in Cameroon were also polymorphic. Multiplex polymerase chain reaction systems were developed with 15 loci for M. fijiensis.