Premium
A microsatellite DNA toolkit for studying population structure in Apis mellifera
Author(s) -
SHAIBI T,
LATTORFF HMG,
MORITZ RFA
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02146.x
Subject(s) - biology , microsatellite , population , genetics , selection (genetic algorithm) , multiplex polymerase chain reaction , evolutionary biology , multiplex , polymerase chain reaction , computational biology , gene , artificial intelligence , computer science , allele , demography , sociology
Abstract We present a set of 18 microsatellite DNA markers that can be run in two multiplex polymerase chain reactions as standard tool for assessing molecular ecological problems in honeybees ( Apis mellifera ). In addition to a set of six unlinked loci testing for classical population genetic parameters, we present three sets of four tightly linked loci, each located on three different chromosomes. These linked markers are useful for determining the number of colonies in a population as well as the parentage of drones and workers. Moreover, the tool kit can test for various modes of natural selection in honeybee populations.