Isolation and characterization of polymorphic microsatellite loci from  Tetratheca ericifolia  (Elaeocarpaceae) | Zendy

MCPHERSON HANNAH | Zendy; PORTER CAROLYN | Zendy; RYMER PAUL D. | Zendy; CRAYN DARREN M. | Zendy; ROSSETTO MAURIZIO | Zendy

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Isolation and characterization of polymorphic microsatellite loci from Tetratheca ericifolia (Elaeocarpaceae)

Author(s) -

MCPHERSON HANNAH,

PORTER CAROLYN,

RYMER PAUL D.,

CRAYN DARREN M.,

ROSSETTO MAURIZIO

Publication year - 2008

Publication title -

molecular ecology resources

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.96

H-Index - 136

eISSN - 1755-0998

pISSN - 1755-098X

DOI - 10.1111/j.1755-0998.2008.02093.x

Subject(s) - biology , microsatellite , loss of heterozygosity , locus (genetics) , genetics , allele , population , evolutionary biology , gene , demography , sociology

We identified 11 informative microsatellite loci in Tetratheca ericifolia from an (AG) n ‐enriched library. Using these loci on 32 individuals from two populations (16 individuals from each), we detected an average of 11.3 alleles per locus (range of five to 21, average expected heterozygosity of 0.728), of which 56% were unique to one population or the other. All loci were amplifiable in seven to 12 of a further 12 species of Tetratheca under the same reaction conditions. The markers will be useful tools for evolutionary studies of this Australian endemic group.

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