Open AccessDestabilized eYFP variants for dynamic gene expression studies in C orynebacterium glutamicum
Author(s) -
Hentschel Eva,
Will Cornelia,
Mustafi Nurije,
Burkovski Andreas,
Rehm Nadine,
Frunzke Julia
Publication year - 2013
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/j.1751-7915.2012.00360.x
Subject(s) - corynebacterium glutamicum , gene , biology , genetics
Summary Fluorescent reporter proteins are widely used for the non‐invasive monitoring of gene expression patterns, but dynamic measurements are hampered by the extremely high stability of GFP and homologue proteins. In this study, we used SsrA ‐mediated peptide tagging for the construction of unstable variants of the GFP derivative eYFP (enhanced yellow fluorescent protein) and applied those for transient gene expression analysis in the industrial platform organism C orynebacterium glutamicum .