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PROTECTION BY THE FLUORIDE ION AGAINST PHTHALOCYANINE‐INDUCED PHOTODYNAMIC KILLING OF CHINESE HAMSTER CELLS
Author(s) -
BenHur Ehud,
Clay Marian E.,
Vicioso Eduardo F.,
Antunez Antonio R.,
Rihter Boris D.,
Kenney Malcolm E.,
Oleinick Nancy L.
Publication year - 1992
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1992.tb04232.x
Subject(s) - phthalocyanine , chinese hamster , photosensitivity , thymidine , chemistry , dna , hamster , dna synthesis , fluoride , photochemistry , biophysics , biochemistry , microbiology and biotechnology , materials science , biology , inorganic chemistry , organic chemistry , optoelectronics
Abstract— When a dilute F ‐ solution was added to a culture of Chinese hamster cells that had been preincubated with an aluminium phthalocyanine sensitizer derived from AlPcCI, the photosensitivity of the cells was markedly reduced compared to control cells not treated with F ‐ . Under the same treatment conditions, the reduction in [ 3 H]thymidine incorporation into cellular DNA caused by light and this sensitizer and the production of DNA‐protein crosslinks caused by light and this sensitizer were also inhibited by F ‐ . In contrast, the killing of Chinese hamster cells, the reduction of thymidine incorporation by the cells, and the production of DNA‐protein crosslinks in the cells caused by the combination of light and either Photofrin II or the silicon phthalocyanine HOSiPcOSi(CH 3 ) 2 (CH 2 ) 3 ‐N(CH 3 ) 2 were not inibited by F ‐ . We conclude that the aluminium phthalocyanine sensitizer used is largely or completely AlPc(OH)(H 2 O), that it is converted to a fluoro complex by F ‐ that this compound probably is a less efficient generator of photochemical damage at a critical cellular target(s) than is AlPc(OH)(H 2 O). The inhibition of thymidine incorporation and DNA‐protein crosslink formation indicates that the effects of F ‐ can be expressed at intracellular sites. It is further concluded that the silicon phthalocyanine sensitizer and Photofrin II do not interact significantly with F ‐ .