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THE RELATIVE CYTOTOXICITY OF(6–4) PHOTOPRODUCTS AND CYCLOBUTANE DIMERS IN MAMMALIAN CELLS *
Author(s) -
Mitchell David L.
Publication year - 1988
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1988.tb02785.x
Subject(s) - cyclobutane , pyrimidine dimer , ultraviolet , dimer , cytotoxicity , dna , chemistry , ultraviolet light , photochemistry , dna damage , somatic cell , dna repair , cell culture , cytotoxic t cell , stereochemistry , biophysics , biology , biochemistry , in vitro , genetics , gene , ring (chemistry) , materials science , organic chemistry , optoelectronics
Abstract— The significance of the pyrimidine(6‐4)pyrimidone photoproduct in mammalian cell killing is considered. Photochemical data indicate that the(6–4) photoproduct is induced at a substantial frequency compared to the cyclobutane dimer and that the action spectra for the induction of both lesions are equivalent. The repair of(6–4) photoproducts in various normal and UV‐hypcrsensitive mammalian cell lines, including several recently derived somatic cell hybrids and transformants, is presented. The sensitivity of these cells to ultraviolet irradiation correlates better with the capacity to repair(6–4) photoproducts than cyclobutane dimers. These data are used to support that idea that the(6–4) photoproduct is one of the major cytotoxic lesions induced in DNA by ultraviolet light.