Premium
A RAPID PROCEDURE FOR THE PURIFICATION OF 124 kDALTON PHYTOCHROME FROM AVENA
Author(s) -
Datta Neeraj,
Roux Stanley J.
Publication year - 1985
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1985.tb03478.x
Subject(s) - phytochrome , avena , chromatography , chemistry , sieve (category theory) , molecular sieve , biochemistry , biology , botany , red light , catalysis , mathematics , combinatorics
— A modification of previously published procedures is described which permits the preparation of high‐purity 124 kDa phytochrome within 14 h. The last 5 h are used for chromatography on a molecular sieve column, so all the handling steps are completed within 9 h. The procedure is simpler and faster than previously published methods.