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ENHANCED REPLICATION OF DAMAGED SV40 DNA IN CARCINOGEN‐TREATED MONKEY CELLS
Author(s) -
Maga Janet A.,
Dixon Kathleen
Publication year - 1984
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1984.tb04620.x
Subject(s) - virus , biology , viral replication , dna replication , virology , dna , ultraviolet light , carcinogen , simian , cell culture , microbiology and biotechnology , chemistry , genetics , photochemistry
Abstract Treatment of mammalian cells with certain chemical or physical carcinogens prior to infection with ultraviolet‐irradiated virus results in enhanced survival or reactivation of the damaged virus. To investigate the molecular basis of this enhanced reactivation (ER), we have examined Simian virus 40 DNA replication in carcinogen‐treated cells. We find that treatment of monkey kidney cells with N‐acetoxy‐2‐acetylamino‐fluorene or UV radiation 24 h prior to infection with ultraviolet‐irradiated Simian virus 40 leads to enhancement of viral DNA replication measured at 36 h after infection by [ 3 H]thymidine incorporation or hybridization. The enhancement of DNA replication is observed when cells are treated from 1 to 60 h before infection or 1 to 16 h after infection. The fact that enhancement is observed also when cells are treated after infection rules out the possibility that enhancement occurs at the level of adsorption or penetration of the virus. Measurements of the time course of viral DNA replication indicate that pretreatment of cells does not alter the time of onset of viral DNA replication. We conclude from these studies that ER of Simian virus 40 occurs at the level of viral DNA replication.