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REPAIR OF ULTRAVIOLET‐DAMAGED TRANSFORMING DNA IN A MISMATCH REPAIR‐DEFICIENT STRAIN OF HAEMOPHILUS INFLUENZAE
Author(s) -
Bagci Hasan,
Stuy Johan H.
Publication year - 1982
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1982.tb02570.x
Subject(s) - dna , dna repair , strain (injury) , haemophilus influenzae , dna mismatch repair , nucleotide excision repair , ultraviolet , biology , microbiology and biotechnology , chemistry , genetics , bacteria , materials science , optoelectronics , anatomy
Abstract— Ultraviolet inactivation of Haemophilus influenzae transforming DNA followed inverse square root kinetics in both mismatch repair‐proficient(hex + ) and deficient (hex‐1) recipients. No DNA concentration effect was seen with UV‐excision repair‐deficient(uvr ‐ ) strains. Low‐efficiency genetic markers remained more sensitive than high‐efficiency ones when they were assayed on excision repair‐deficienthex + uvr ‐ strains. They were equally resistant whenhex + uvr ‐ recipients were used. We explain this by assuming that recombinational repair of UV lesions in the donor strand and mismatch repair of the recipient strand may overlap and cause double strand interruptions. This will eliminate low‐efficiency transformants.