Premium
U.V. INDUCED CONFORMATIONAL CHANGES IN TRANSFER RNA
Author(s) -
SARIN P. S.,
JOHNS H. E.
Publication year - 1968
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1968.tb08006.x
Subject(s) - transfer rna , rna , chemistry , conformational change , acceptor , function (biology) , biophysics , escherichia coli , stereochemistry , biochemistry , biology , physics , microbiology and biotechnology , gene , condensed matter physics
— The quantum yields for the u.v. inactivation of the amino acid acceptor function of E. coli transfer RNA (for val, phe and lys) and for the loss of its conformation, as a function of exposure, have been determined following irradiation at 280, 265 and 254 nm. Our results suggest that u.v. damage produces a change in the conformation of transfer RNA which in turn inactivates it, and that the anticodon is not the u.v. sensitive site. Calculations indicate that a small number of photoproducts inactivate the transfer RNA.