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Use of an enzyme‐linked immunosorbent assay in a bovine brucellosis eradication program
Author(s) -
LEE C. CARGILL K.,
CLARKE I.
Publication year - 1985
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/j.1751-0813.1985.tb14233.x
Subject(s) - complement fixation test , brucellosis , bovine brucellosis , herd , haemolysis , brucella abortus , veterinary medicine , serology , virology , biology , rose bengal , medicine , antibody , immunology , genetics
SUMMARY: An enzyme‐linked immunosorbent assay (ELISA) was developed and was compared wih the complement fixation test (CFT) in a bovine brucellosis eradication program. The ELISA detected significantly more reactors than the CFT in both strain 19 vaccinated infected herds (1.79% versus 1.14%) and non‐vaccinated infected herds (4.2% versus 3.59%) but not in either vaccinated or non‐vaccinated brucella‐free herds. The specificity for both tests in brucella‐free herds was greater than 0.998. The specificity and sensitivity of the ELISA were compared with those of 3 other tests (the Rose Bengal test; the indirect haemolysis test [IHLT] and the CFT) on serum from 151 animals cultured at slaughter. The calculated specificity of the ELSA in this infected group was lower than for both the CFT and the IHLT (0.58 versus 0.67 versus 0.75). The sensitivity however was much greater (1.0 versus 0.73 versus 0.71). The value of the ELSA when used in an eradication program is discussed.