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Lactococcus lactis ssp. lactis Inhibits the Proliferation of SNU‐1 Human Stomach Cancer Cells through Induction of G0/G1 Cell Cycle Arrest and Apoptosis via p53 and p21 Expression
Author(s) -
Kim Seo Young,
Kim JongEun,
Lee Ki Won,
Lee Hyong Joo
Publication year - 2009
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2009.04721.x
Subject(s) - apoptosis , cell cycle , cell growth , lactococcus lactis , cell cycle checkpoint , microbiology and biotechnology , cancer cell , biology , cyclin d1 , growth inhibition , g1 phase , chemistry , cancer , biochemistry , genetics , lactic acid , bacteria
We investigated the antiproliferative effects of the cytoplasmic fraction of Lactococcus lactis ssp . lactis (L.lac CF) on the SNU‐1 human stomach cancer cell line. The proliferation of SNU‐1 cells was inhibited by treatment with L.lac CF in a time‐ and dose‐dependent manner. L.lac CF caused G0/G1 cell cycle arrest, which was associated with an increase in p53 and p21 expression, the reduction of cyclin D1 expression, and retinoblastoma protein phosphorylation. L.lac CF induced apoptosis in SNU‐1 cells, as demonstrated by increased nucleus condensation and a sub‐G1 peak. Caspase‐3 activation, the induction of p53, and the downregulation of Bcl‐2 were also observed in L.lac CF‐treated cells. Thus, the inhibitory effect of L.lac CF on SNU‐1 cell growth is mainly attributable to the induction of G0/G1 cell cycle arrest and apoptosis.
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