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Gamma Structural Variants Differentially Regulate Na,K‐ATPase Properties
Author(s) -
ARYSTARKHOVA ELENA,
WETZEL RANDALL K.
Publication year - 2003
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2003.tb07223.x
Subject(s) - splice , protein subunit , gamma subunit , atpase , transfection , nephron , microbiology and biotechnology , chemistry , alternative splicing , gene , kidney , biology , enzyme , gene isoform , biochemistry , genetics
A bstract : Renal Na,K‐ATPase is tightly bound to a small regulatory protein, the gamma subunit (FXYD2). In rat, it occurs in two splice variants with different N‐termini. Immunolocalization on kidney sections revealed distinct distribution of the γ splice variants along the rat nephron. Where coexpressed, they coimmunoprecipitated with each other along with the alpha subunit, suggesting assembly in oligomeric complexes. Functional consequences of association with gamma were monitored in stably transfected NRK‐52E cells. The outcome was that splice variants can differentially modulate the major intrinsic properties of the Na,K‐ATPase under normal and stress‐related conditions. The data imply an adaptive physiological mechanism of regulation of renal Na,K‐ATPase through modulation of pump properties, gene expression, or both.