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Global Analysis of Brucella melitensis Proteomes
Author(s) -
MUJER CESAR V.,
WAGNER MARY ANN,
ESCHENBRENNER MICHEL,
HORN TROY,
KRAYCER JO ANN,
REDKAR RAJENDRA,
HAGIUS SUE,
ELZER PHILIP,
DELVECCHIO VITO G.
Publication year - 2002
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2002.tb04358.x
Subject(s) - brucella melitensis , virulence , proteome , brucellosis , brucella , biology , microbiology and biotechnology , identification (biology) , brucellaceae , virology , genetics , gene , ecology
A bstract :Brucella melitensis is a facultative, intracellular, gram‐negative cocco‐bacillus that causes Malta fever in humans and brucellosis in animals. There are at least six species in the genus, and the disease is classified as zoonotic because several species infect humans. Using 2‐D gel electrophoresis and mass spectrometry, we have initiated (i) a comprehensive mapping and identification of all the expressed proteins of B. melitensis virulent strain 16M, and (ii) a comparative study of its proteome with the attentuated vaccinal strain Rev 1. Comprehensive proteome maps of all six Brucella species will be generated in order to obtain vital information for vaccine development, identification of pathogenicity islands, and establishment of host specificity and evolutionary relatedness.