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Organization of the Rat PACAP Gene
Author(s) -
WHITE SHERYL L.,
MAY VICTOR,
BRAAS KAREN M.
Publication year - 2000
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2000.tb06996.x
Subject(s) - gene , chemistry , microbiology and biotechnology , biology , biochemistry
The rat PACAP gene has been isolated, cloned, and sequenced. The gene organization is very similar to that of the mouse and human PACAP genes. An analysis of the 5'-UTR in proPACAP mRNAs indicates that multiple transcripts are generated by using different splice sites within exons 1A, 1B, and 2 to form the 5'-UTR. Analysis of PACAP promoter activity in PC12 cells suggests that forskolin responsiveness of the gene is dependent on elements located between -77 and -413 bp from the transcription start site.