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Quantitative Proteome Analysis: Methods and Applications
Author(s) -
AEBERSOLD RUEDI,
RIST BEATE,
GYGI STEVEN P.
Publication year - 2000
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2000.tb06865.x
Subject(s) - proteome , computational biology , function (biology) , protein expression , computer science , quantitative analysis (chemistry) , bioinformatics , biology , gene , genetics , chemistry , chromatography
A bstract : With the completion of a rapidly increasing number of complete genomic sequences, much attention is currently focused on how the information contained in sequence databases might be interpreted in terms of the structure, function, and control of biological systems. Quantitative proteome analysis, the global analysis of protein expression, has been proposed as a method to study steady‐state gene expression and perturbation‐induced changes. Here, we discuss the rationale for quantitative proteome analysis, highlight the limitations in the current standard technology, and introduce a new experimental approach to quantitative proteome analysis.