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Detection of DNA in the Plasma of Septic Patients
Author(s) -
MARTINS GLORIA ADRIANA,
KAWAMURA MARCIA TIE,
CARVALHO MARIA DA GLORIA DA COSTA
Publication year - 2000
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2000.tb06603.x
Subject(s) - septic arthritis , dna , rheumatoid arthritis , medicine , dot blot , gene , immunology , virology , biology , arthritis , genetics
A bstract : Small amounts of plasma‐free DNA have been observed both in healthy individuals and in patients with various diseases such as systemic lupus erythematosus, rheumatoid arthritis, viral hepatitis, and cancer. This communication demonstrates that septic patients also release DNA in plasma. After DNA extraction from plasma, exon 1 of the K‐ras gene was amplified by PCR and products were analyzed by dot‐blot hybridization. Plasmas from polytraumatic patients and control healthy individuals were used for comparisons with septic patients. Our results show that septic patients present DNA in their plasma. As far as we know, this is the first evidence of circulating DNA in septic patients.