Premium
Involvement of Nuclear Receptors in the Enhanced IL‐2 Production by Melatonin in Jurkat Cells
Author(s) -
GUERRERO JUAN M.,
POZO DAVID,
GARCÍAMAURIÑO SOFÍA,
OSUNA C.,
MOLINERO P.,
CALVO JUAN R.
Publication year - 2000
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2000.tb05404.x
Subject(s) - jurkat cells , melatonin , receptor , microbiology and biotechnology , nuclear receptor , melatonin receptor , biology , chemistry , t cell , biochemistry , endocrinology , immunology , immune system , transcription factor , gene
A bstract : This report shows that melatonin enhances IL‐2 production by Jurkat cells via a nuclear receptor‐mediated mechanism. Jurkat cells express nuclear (RZRα, RORα1, and RORα2) and membrane (mt1) melatonin receptors, and melatonin binds to Jurkat nuclei and membranes with the same affinity described for human peripheral blood mononuclear cells (PBMCs). Melatonin enhances IL‐2 production by Jurkat cells activated by either phytohemagglutinin (PHA) or phorbol myristate acetate (PMA). PHA activation of Jurkat cells does not change the profile of melatonin receptor expression; on the contrary, PMA activation negatively regulates the mt1 receptor. In the absence of the membrane receptor, melatonin still activates the IL‐2 production. These results show that the expression of the nuclear melatonin receptor is sufficient for melatonin to activate IL‐2 production by Jurkat cells.