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Identification and Characterization of RNA Editing Events within the 5‐HT 2C Receptor a
Author(s) -
NISWENDER C. M.,
SANDERSBUSH E.,
EMESON R. B.
Publication year - 1998
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1998.tb10171.x
Subject(s) - rna editing , rna , biology , 5 ht5a receptor , intron , messenger rna , post transcriptional modification , microbiology and biotechnology , genetics , biochemistry , receptor , gene
ABSTRACT: RNA editing is a post‐transcriptional modification that generates an RNA transcript with a nucleotide sequence different from its gene. We have recently discovered RNA editing events, involving the conversion of adenosine bases to inosine residues, within the RNA encoding the serotonin 2C (5‐HT 2C ) receptor. 1 Editing events at four major positions, termed A, B, C and D, as well as one minor site termed C′, are predicted to alter amino acids within the second intracellular loop of the G‐protein coupled 5‐HT 2C receptor. Editing is mediated by at least two members of a family of adenosine deaminases and is contingent upon the presence of an extensive RNA duplex structure formed by exonic and intronic sequences of 5‐HT 2C receptor precursor messenger RNA (pre‐mRNA). This critical secondary structure has been observed within brain pre‐mRNA derived from four species; the isolation of edited 5‐HT 2C receptor transcripts from these samples further confirms the evolutionary conservation of this RNA processing event. Among members of the 5‐HT 2 receptor family, editing within second intracellular loop RNA is unique to the 5‐HT 2C receptor. Editing within the 5‐HT 2C receptor generates receptor isoforms that differ in their ability to interact with the phospholipase C signaling cascade in a transfected cell line, suggesting that this RNA processing event may contribute to the modulation of serotonergic neurotransmission in the central nervous system.