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Effect of sampling method and incubation temperature on fungal culture in canine sinonasal aspergillosis
Author(s) -
Billen F.,
Clercx C.,
Le Garérrès A.,
Massart L.,
Mig B.,
Peeters D.
Publication year - 2009
Publication title -
journal of small animal practice
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.7
H-Index - 67
eISSN - 1748-5827
pISSN - 0022-4510
DOI - 10.1111/j.1748-5827.2008.00672.x
Subject(s) - aspergillosis , medicine , fungal growth , incubation , pathology , mycosis , aspergillus , mucous membrane of nose , microbiology and biotechnology , surgery , biology , immunology , biochemistry
O bjectives :To evaluate the most appropriate sampling procedure and the effect of incubation temperature on fungal culture in the diagnosis of canine sinonasal aspergillosis (SNA).M ethods :Sixteen dogs with SNA and 20 dogs with non‐fungal nasal disease entered a prospective study. Nasal secretions and mucosal biopsies were collected in all dogs. Fungal plaques were also sampled in dogs with SNA. Each specimen was taken in duplicate from each dog and incubated at room temperature and 37°C.R esults :In dogs with SNA, nasal secretions, mucosal biopsies and fungal plaques yielded fungal growth at room temperature in one, one and seven dogs, respectively, whereas fungal growth was obtained at 37°C in three, 12 and 14 dogs, respectively. No specimen collected from any dog with non‐fungal nasal disease yielded fungal growth at room temperature or at 37°C.C linical S ignificance :The diagnosis of canine SNA is more likely to be confirmed following culture of mucosal biopsies or fungal plaques than nasal secretions sampled blindly with swabs. Incubating cultures at 37°C is more likely to provide a diagnostic outcome than when samples are cultured at room temperature. Fungal culture of nasal specimens has good specificity for the diagnosis of SNA in dogs.