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OCCURRENCE OF AFLATOXIN M 1 IN RAW MILK FROM SOUTH KOREA WINTER SEASONS USING AN IMMUNOAFFINITY COLUMN AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Author(s) -
KIM HO JIN,
LEE JI EUN,
KWAK BYUNGMAN,
AHN JANGHYUK,
JEONG SEUNGHWAN
Publication year - 2010
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.2010.00243.x
Subject(s) - aflatoxin , contamination , raw milk , atomic force microscopy , high performance liquid chromatography , chromatography , food contaminant , chemistry , dairy industry , environmental science , food science , materials science , biology , nanotechnology , ecology
The contamination level of aflatoxin M 1 (AFM 1 ) in raw milk samples produced in South Korea during the winter season was investigated using immunoaffinity column chromatography and high performance liquid chromatography equipped with a fluorescence detector. Among the test samples collected from 100 cattle ranches in 2008 and 2009, none were contaminated with AFM 1 over the maximum level (0.5 µg/L) set by South Korea and U.S. regulatory agencies. Seven percent of the tested samples obtained in 2008 were not contaminated with AFM 1 ; however, this increased to 34% for samples obtained in 2009. The percent of samples with an AFM 1 concentration in the range of 0.05–0.10 µg/L decreased from 21% in 2008 to 4% in 2009. In this study, the level of AFM 1 in raw milk was determined to be very low and safe for market milk process.PRACTICAL APPLICATIONS This study, the first time in South Korea, contamination of aflatoxin M 1 (AFM 1 ) in raw milk, during 2 years seasons was investigated using immunoaffinity column and high performance liquid chromatography. This was a large survey for AFM 1 level in the raw milk samples from 100 dairy ranches. The result can be used as reference data to understand the current occurrence of AFM 1 and estimate the contamination levels of AFM 1 in various dairy products in South Korea.