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EFFECTS OF HELPER PROTEINS ENCODED BY p 19 AND orf 1‐ orf 2 GENES ON Cyt1Aa PROTEIN EXPRESSION IN ACRYSTALLIFEROUS STRAIN OF BACILLUS THURINGIENSIS
Author(s) -
Tang Mujin,
Zeng Shaoling,
Chen Jianwu,
Shi Yongxia,
Xu Wei,
Yuan Meijin,
Pang Yi
Publication year - 2003
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/j.1744-7917.2003.tb00386.x
Subject(s) - bacillus thuringiensis , biology , strain (injury) , gene , microbiology and biotechnology , open reading frame , genetics , bacteria , peptide sequence , anatomy
A series of plasmids were constructed to examine the effects of p 19 and orf 1‐ orf 2 genes from Bacillus thuringiensis on Cyt1Aa synthesis and inclusion formation. The plasmids expressed the cyt1Aa gene along with either p 19 or orf 1‐ orf 2, or each of them coordinatively with p 20 in the acrystalliferous strain of B. thuringiensis subsp. israelensis 4Q7. No effect on the expression of Cyt1Aa protein was found when P19 or Orf1‐Orf2 co‐expressed with Cyt1Aa. However, when including p 20 gene, the constructs with p 19 or orf 1‐ orf 2 gene produced lower yield of Cyt1Aa proteins than without p 19 or orf 1‐ orf 2 gene. Electron microscopy observation and bioassay showed that P19 and Orf1‐Orf2 have no influence on the crystal size and toxicity of Cyt1Aa protein. It is presumed that P19 and Orf1‐Orf2 might have negative effects on Cyt1Aa synthesis in B. thuringiensis.